Determination of As(III) and As(V) in bacterial culture by electro-thermal atomic absorption spectrometry (ETAAS) after separation with an anion exchange mini-column

MAIZEL, DANIELA; BALVERDI, PILAR; BALVERDI, CECILIA; SALES, ADRIANA M.; FERRERO, MARCELA A.

One Century of the Discovery of Arsenicosis in Latin America (1914-2014) As2014: Proceedings of the 5th International Congress on Arsenic in the Environment

Taylor & Francis Group

Lugar: Londres; Año: 2014; p. 183 - 184

A simple approach is described for the separation and determination of inorganic arsenic species using solid phase extraction and electrothermal atomic absorption spectrometry (ETAAS). A Dowex 2x10 anion exchange mini-column was used to separate As(III) and As(V) from a minimal culture medium, LB25 clean medium and LB25 after bacterial growth. The chemical (pH, type and concentration of eluent) and physical (flow rate of sample and eluent) parameters affecting the separation were studied. Under optimized conditions, As(V) showed a strong affinity for the mini-column, while As(III) was collected in the effluent. As(V) was recovered by elution with 0.8 M hydrochloric acid. The influence of different culture media on the separation of As(III) and As(V) was also evaluated. Inorganic As species were detected in the supernatants of free-cells from a growth culture of bacteria isolated from drinking water wells of the Tucumán province.