PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
capítulos de libros
Título:
Tailoring of industrial products by submerged fermentation
Autor/es:
PERA L.M; LUNA FLAVIA; CASTRO, GR; BAIGORÍ M
Libro:
Industrial Fermentation: Food Processes, Nutrient Sources and Production Strategies
Editorial:
Nova Science
Referencias:
Año: 2010; p. 1 - 31
Resumen:
Microbial cultivation processes for production of goods is ancient and well-known
technology. However, over the last 10 years it use has increased rapidly because of the
combination of a more deep knowledge of cellular metabolic pathways, changes in
operational costs, product quality requirements and additionally as green or
environmentally friendly technology among others.
Researchers are now taking advantage of different cultivation strategies, from batch
controlled procedures in one side to continuous culture systems. Particularly, continuous
culture procedures combined with metabolic flux analysis and molecular biology
techniques, are used for pathway manipulation during growth in steady state conditions,
which should yield new insights into the physiology of industrial organisms, as well as
leading to increased productivity potential. The determination of metabolic fluxes in-vivoin-vivo
and subsequent metabolic flux analyses are best conducted under steady-state conditions.
Removal of transient growth effects present in batch cultures allows more realistic
estimation of metabolic pathway deletions or enhancements effects in continuous
cultures. Also, the use of metabolite measurements coupled with the stoichiometry of
reactions has been applied mainly in metabolic networks validations.
On the other hand, new concepts had been developed as the medium engineering
area. This approach suggests the feasibility of producing new industrially relevant
molecules having different applications simply by controlling fermentations parameters.
This chapter highlights recent findings and new strategies in tailoring of some relevant
industrial products by submerged fermentation. As examples we want to show significant
applications such as lipase, and nitrogen-fixing microorganism production.