PROIMI   05436
PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLOGICOS
Unidad Ejecutora - UE
artículos
Título:
Copper removal ability by Streptomyces strains with dissimilar growth patterns and endowed with cupric reductase activity
Autor/es:
VIRGINIA HELENA ALBARRACIN; ANA LUCIA AVILA; AMOROSO M. J.; ABATE C. M.
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
Blackwell Publishing Ltd
Referencias:
Lugar: Inglaterra; Año: 2008 vol. 288 p. 141 - 148
ISSN:
0378-1097
Resumen:
Morphological, physiological and molecular characterization of three copperresistant
actinobacterial strains (AB2A, AB3 and AB5A) isolated from copperpolluted
sediments of a drainage channel showed that they belonged to the genus
Streptomyces. These characteristics plus their distinctive copper resistance phenotypes
revealed considerable divergence among the isolates. Highly dissimilar
growth patterns and copper removal efficiency were observed for the selected. These characteristics plus their distinctive copper resistance phenotypes
revealed considerable divergence among the isolates. Highly dissimilar
growth patterns and copper removal efficiency were observed for the selected
Streptomyces strains grown on minimal medium (MM) added with 0.5mM of
copper sulfate (MMCu). Strain AB2A showed an early mechanism of copper
uptake/retention (80% until day 3), followed by a drastic metal efflux process (days
57). In contrast, Streptomyces sp. AB3 and AB5A showed only copper retention
phenotypes under the same culture conditions. Particularly, Streptomyces sp. AB5A
showed a better efficiency in copper removal (94%), although a longer lag phase
was observed for this microorganism grown for 7 days in MMCu. Cupric reductase
activity was detected in both copper-adapted cells and nonadapted cells of all three
strains but this activity was up to 100-fold higher in preadapted cells ofstrains grown on minimal medium (MM) added with 0.5mM of
copper sulfate (MMCu). Strain AB2A showed an early mechanism of copper
uptake/retention (80% until day 3), followed by a drastic metal efflux process (days
57). In contrast, Streptomyces sp. AB3 and AB5A showed only copper retention
phenotypes under the same culture conditions. Particularly, Streptomyces sp. AB5A
showed a better efficiency in copper removal (94%), although a longer lag phase
was observed for this microorganism grown for 7 days in MMCu. Cupric reductase
activity was detected in both copper-adapted cells and nonadapted cells of all three
strains but this activity was up to 100-fold higher in preadapted cells ofCu). Strain AB2A showed an early mechanism of copper
uptake/retention (80% until day 3), followed by a drastic metal efflux process (days
57). In contrast, Streptomyces sp. AB3 and AB5A showed only copper retention
phenotypes under the same culture conditions. Particularly, Streptomyces sp. AB5A
showed a better efficiency in copper removal (94%), although a longer lag phase
was observed for this microorganism grown for 7 days in MMCu. Cupric reductase
activity was detected in both copper-adapted cells and nonadapted cells of all three
strains but this activity was up to 100-fold higher in preadapted cells ofStreptomyces sp. AB3 and AB5A showed only copper retention
phenotypes under the same culture conditions. Particularly, Streptomyces sp. AB5A
showed a better efficiency in copper removal (94%), although a longer lag phase
was observed for this microorganism grown for 7 days in MMCu. Cupric reductase
activity was detected in both copper-adapted cells and nonadapted cells of all three
strains but this activity was up to 100-fold higher in preadapted cells ofStreptomyces sp. AB5A
showed a better efficiency in copper removal (94%), although a longer lag phase
was observed for this microorganism grown for 7 days in MMCu. Cupric reductase
activity was detected in both copper-adapted cells and nonadapted cells of all three
strains but this activity was up to 100-fold higher in preadapted cells ofCu. Cupric reductase
activity was detected in both copper-adapted cells and nonadapted cells of all three
strains but this activity was up to 100-fold higher in preadapted cells of
Streptomyces sp. AB2A. To our knowledge, this is the first time that cupric
reductase activity was demonstrated in Streptomyces strains.sp. AB2A. To our knowledge, this is the first time that cupric
reductase activity was demonstrated in Streptomyces strains.Streptomyces strains.