Development of an antioxidant phytoextract of Lantana grisebachii with lymphoprotective activity against in vitro arsenic toxicity.. ID 416761

SORIA, ELIO A; QUIROGA, PATRICIA L; ALBRECHT, C; RAMOS ELIZAGARAY, IS; CANTERO, JUAN J; BONGIOVANNI, GUILLERMINA A.

Advances in Pharmacological Sciences

Hindawi Publishing Corporation

Año: 2014 vol. 2014

1687-6334

Phytochemicals have been presumed to possess prophylactic and curative properties in several pathologies, such as arsenic- (As-) induced immunosuppression. Our aim was to discover a lymphoprotective extract from Lantana grisebachii Stuck. (Verbenaceae) (LG). We assessed its bioactivity and chemical composition using cell-based assays. Fractions produced from a hexane extract acutely induced nitrite formation in T-activated cell cultures (𝑃 < 0.0001). Water extraction released a fraction lacking nitrite inducing activity in both lymphocyte types. Aqueous LG was found to be safe in proliferated and proliferating cells. The infusion-derived extract presented better antioxidant capacity in proportion to phenolic amount in lymphocytes (infusive LG- 1i at 100 𝜇g/mL), which protected them against in vitro As-induced lymphotoxicity (𝑃 < 0.0001). This infusive LG phytoextract contained 10.23 ± 0.43 mg/g of phenolics, with 58.46% being flavonoids. Among the phenolics, the only predominant compound was 0.723mg of chlorogenic acid per gram of dry plant, in addition to 10 unknown minor compounds. A fatty acid profile was assessed. It contained one-third of saturated fatty acids, one-third of 𝜔9, followed by 𝜔6 (∼24%) and 𝜔3 (∼4%), and scarce 𝜔7. Summing up, L. grisebachii was a source of bioactive and lymphoprotective compounds, which could counteract As-toxicity. This supports its phytomedical use and research in order to reduce As-related dysfunctions.