Evaluation of different lipases in the esterification reaction of the sn-2 position of 1,3-diglycerides

SANCHEZ D.; TONETTO G.; FERREIRA M.

Buzios

Workshop; VII Workshop on Biocatalysis and Biotransformation; 2014

Universidade Federal do Rio de Janeiro

Structured triglycerides (ST) containing a mixture of medium chain fatty acids (MCFA) and long-chain fatty acids (LCFA) in the same molecule have been designed for medical and nutritional purposes. The placement of a long (L) chain fatty acid at the sn-2 position of glyceryl carbons, particularly if it is saturated, increases the digestibility of the MLM lipid, being in the sn-1 and sn-3 positions a medium (M) chain fatty acid [1-2].In this work different lipases were choose to evaluate their activity in the esterification of the sn-2 position of 1,3-dicaprin with palmitic acid. Different methodologies for controlling water in the reaction medium were proposed.