Quantitative Determination of Intracellular PHA in Bacillus megaterium BBST4 Strain Using Mid FTIR Spectroscopy

M.A. PORRAS; M.A. CUBITTO; M.A. VILLAR

Porto de Galinhas, RE

Simposio; XIV Latin American Simposium on Polymers, SLAP14 and XII Ibero American Congress on Polymers, CIP14; 2014

Associação Brasileira de Polímeros

Poly (beta-hydroxyalkanoate)s, PHAs, constitute a family of natural biodegradable polyesters produced by bacteria as reserve of carbon and energy source in intracytoplasmic granules. They have similar properties to non-biodegradable petrochemical-based polymers. A major limiting in the widespread use of PHAs is in the production cost thereof. In this work, the potential of production of PHA of Bacillus megaterium BBST4 strain, cultured in a minimal medium of low cost, using glycerol as carbon sources and urea as nitrogen source, is correlated through multivariate statistical analysis to developed a rapid quantitative determination technique of extractable intracellular biopolymer in the strain.