INVESTIGADORES
PIECKENSTAIN Fernando Luis
congresos y reuniones científicas
Título:
Polyamine metabolism of tobacco plants infected by Sclerotinia sclerotiorum
Autor/es:
MARINA M, E RIVAS, A GÁRRIZ, MC DALMASSO, FL PIECKENSTAIN, OA RUIZ
Lugar:
Bariloche, Argentina
Reunión:
Congreso; XXXIX Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas; 2003
Institución organizadora:
Sociedad Argentina de Investigaciones Bioquímicas
Resumen:
Polyamines are polycationic compounds that are found in all living
organisms. In plants, they have been shown to be essential for
growth and developmental processes, and recently, a defense
related role was suggested. Regarding this, high levels of spermine
were observed in tobacco plants after TMV infection and spermine
itself was able to induce the expression of pathogenesis-related
proteins in a salicylic-independent way. Furthermore, an increase
of polyamines in plants infected by biotrophic fungi has been
reported but information about nechrotrophic fungi is lacking This
work was aimed to evaluate the role of polyamines in tobacco
plants in response to the infection of the nechrotrophic fungus
Sclerotinia sclerotiorum, the most important pathogen of cultivated
sunflower in Argentina. Tobacco plants inoculated with S.
sclerotiorum mycelium showed higher levels of putrescine than
controls in whole tobacco leaves, but when intercellular spaces
was studied considerable changes in putrescine, spermidine and
spermine was observed. In order to evaluate the specific role of
each polyamine, we infiltrated tobacco leaf discs with solutions
of these compounds before the inoculation. In this case, polyamine
infiltration resulted in an increase of necrotic area of inoculated
leaf discs, suggesting that either polyamines are deleterious to
defense response of tobacco plants or they are able to support
fungal development. The probable role of polyamines in tobacco-
S. sclerotiorum interaction will be discussed.