Polyamine catabolism, hydrogen peroxide production and RBOH oxidases in the Arabidopsis thaliana- Pseudomonas interaction

JASSO ROBLES FI; GONZALEZ ME; PIECKENSTAIN FL; RAMÍREZ-GARCÍA JM; GUERRERO-GONZÁLEZ ML; JIMÉNEZ-BREMONT JF; RODRÍGUEZ-KESSLER M

Puerto Vallarta

Congreso; VVII Plant Biochemistry and Molecular Biology Congress & México-USA Symposium; 2021

Polyamines(PAs) are low molecular weight aliphatic polycations, with important roles in plant growth, development and stress responses. Polyamine oxidases(PAO) are FAD- dependent enzymes that catabolize a variety of higher PAs such as spermidine, spermine and thermospermine. Hydrogen peroxide(H2O2) is an important product of PA catabolism, thereby linking PA degradation with cell signaling, defense responses and programmed cell death. Herein, the importance of PA catabolism regulation and the production of H2O2 in biotrophic(maize-Ustilago maydis) and hemibiotrophic(Arabidopsis-Pseudomonas) interactions are presented. In both cases, a differential expression of members of the maize and Arabidopsis PAO gene families were noticed. In maize, our data suggest that H2O2 produced through PA catabolism by ZmPAO1(encoding en extracellular enzyme) plays an important role in tumor development during the maize-U. maydis interaction. In the case of Arabidopsis, AtPAO1 and AtPAO2 genes(encoding citosolic and peroxisomal enzymes, respectively) are important in defense responses against Pseudomonas. Alteration in PA catabolism(using T-DNA insertional lines) affects ROS production and the activities of RBOH, SOD and catalase enzymes. Our data indicate that PA catabolism is modulated differentially in monocot and dicot in plant-pathogen interactions.