Ultradeformable archaeosomes as new topical adjuvants

HIGA LH; SCHILRREFF P; PEREZ AP; IRIARTE MA; RONCAGLIA DI; MORILLA MJ; ROMERO EL

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE

ELSEVIER SCIENCE BV

Año: 2012 vol. 8 p. 1319 - 1328

1549-9634

Ultradeformable archaeosomes (UDA) are vesicles made of soybean phosphatidylcholine (SPC),sodium cholate (NaChol) and polar lipids from Halorubrum tebenquichense (3:1:3 wt/wt). Whileultradeformable liposomes (UDL, made of SPC and NaChol at 6:1 wt/wt) and UDA were neithercaptured nor caused cytotoxicity on keratinocytes, UDA was avidly captured by macrophages, beingtheir viability reduced by 0.4-1.6mg/ml phospholipids, to 60-25 %. Instead, UDL were poorly capturedand caused no toxicity. Balb/C mice immunized by the topical route with four doses of ovalbumin(OVA)-loaded UDA, at 75 g OVA/600 g phospholipids (125 nm mean size and -42 mV Z potential),induced IgG titers ten to one hundred folds higher than those immunized with OVA loaded-UDL at thesame dosage. Both matrices penetrate to the same skin depth (nearly 10 m after 1h on excisedhuman skin), being the higher topical adjuvancy and higher phagocytic uptake of UDA related to itsglycolipid content.