Preparation of electrospun polyurethane membranes for immobilization of peroxidases

D.A. MORALES URREA; P.C. CARACCIOLO; P.M. HAURE; E.M. CONTRERAS

mar del plata

Congreso; XVI Simposio Latinoamericano de Polímeros (SLAP 2018) y XIV Congreso Iberoamericano de Polímeros (CIP 2018); 2018

INTEMA

The enzyme peroxidase is well known for its capacity to decolorize textile effluents in the presence of hydrogen peroxide1. The immobilization of enzymes shows great perspective due to the possibility of recycle and improved stability2. However, the activity of the enzyme is affected by the structure of the supporting material. Electrospinning produces sub-micron polymer fibers that form membranes with extremely high surface areas on which enzymes can be immobilized covalently3, thus increasing the catalytic activity of the biocatalyst. In this study, polyurethane (PUR) nanofibers produced via electrospinning were used for the immobilization of a commercial horseradish peroxidase (HRP) to catalyse the decolorization of orange II (OII) with hydrogen peroxide (P).