Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA

OYHENART, JORGE; MORERO, MARIANA

ARCHIVES OF MICROBIOLOGY

SPRINGER

Año: 2022 vol. 204

0302-8933

An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplifica- tion (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60min and helped distinguish positives with less difficulty than visual inspection.