Monolithic microcolumns for on-line solid-phase extraction capillary electrophoresis

VIZIOLI NM; GRASSELLI M; CARDUCCI CN

Mar del Plata, Argentina

Conferencia; 8th Latin American Symposium on Biomedical, Biopharmaceutical and Industrial Applications of Capillary Electrophoresis and Microchip Technology, LACE 2002; 2002

The interest in the development and application of on-line preconcentration and micro reactions in capillary electrophoresis is growing rapidly. Using selective adsorbents properly bound to a solid support, affinity ligands can selectively adsorb target analytes from a sample at a wide range of concentrations. Different materials have been used as solid supports. In many cases, the devices contained some type of porous particles packed between two frit structures. Monolithic materials recently employed for the preparation of capillary electrochromatography columns, seem to be promising media for the design of preconcentration microchambers. In this presentation, a monolithic microcolumn for on-line solid-phase extraction with capillary electrophoresis will be described. The synthesis of the support was carried out by radiation copolymerization of diethylene glycol dimethacrylate and glycidylmethacryLate with a 60CO gamma source. The epoxy groups were funtionalized in order to be used for the retention of peptides by affinity bonding to immobilized copper ions.  These fritless devices are simple to prepare and their characteristics allow them to overcome the technical difficulties related with packed microcolumns.   They also show a highly reproducible performance.