Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography

GRELA D; ZANNONI, V; VIZIOLI, NM

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017 vol. 130 p. 153 - 156

0026-265X

In this work, the interaction between synthetic bioactive peptides and polymeric nanoparticles (NPs) used aspseudostationary phase in capillary electrochromatography was studied. NPs were prepared from methacrylicacid and ethylenglycol dimethacrylate with benzoyl peroxide by utilizing a precipitation polymerization technique.The reaction was monitored by infrared spectroscopy. Polymer characterization was performed by dynamiclight scattering and transmission electron microscopy. Experimental running conditions were tested,including organic solvent proportion in the background electrolyte, capillary conditioning, applied voltage, sampleintroduction amount, and how NPs were incorporated into the system. A continuous full filling technique inwhich the NPswere suspended in the entire electrolyte volume as well as a conventional partial filling techniquewere used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides.The interaction between analytes and NPs was found to be predominantly ionic.filling technique inwhich the NPswere suspended in the entire electrolyte volume as well as a conventional partial filling techniquewere used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides.The interaction between analytes and NPs was found to be predominantly ionic.filling techniquewere used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides.The interaction between analytes and NPs was found to be predominantly ionic.