IBCN   20355
INSTITUTO DE BIOLOGIA CELULAR Y NEUROCIENCIA "PROFESOR EDUARDO DE ROBERTIS"
Unidad Ejecutora - UE
artículos
Título:
The expression of NMDA receptor subunits in cerebral cortex and hippocampus is differentially increased by administration of Endobain E, a Na+, K+-ATPase inhibitor
Autor/es:
M. G. BERSIER; C. PEÑA; G. RODRÍGUEZ DE LORES ARNAIZ
Revista:
Neurochemical Research
Editorial:
Springer
Referencias:
Lugar: Nueva York, EE.UU.; Año: 2008 vol. 33 p. 66 - 72
Resumen:
Previous studies showed that endobain E, an
endogenous Na+, K+-ATPase inhibitor, decreases dizocilpine
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endogenous Na+, K+-ATPase inhibitor, decreases dizocilpine
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endogenous Na+, K+-ATPase inhibitor, decreases dizocilpine
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
binding to NMDA receptor in isolated membranes.
The effect of endobain E on expression of NMDA receptor
subunits in membranes of rat cerebral cortex and hippocampus
was analyzed by Western blot. Two days after
administration of 10 ll endobain E (1 ll = 29 mg fresh
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
tissue) NR1 subunit expression enhanced 5-fold and 2.5-
fold in cerebral cortex and hippocampus, respectively.
NR2A subunit expression increased 2-fold in cerebral
cortex and 1.5-fold in hippocampus. The level of NR2B
subunit raised 3-fold in cerebral cortex but remained
unaltered in hippocampus. NR2C subunit expression was
unaffected in either area. NR2D subunit enhanced 1.6 and
2.1-fold for cerebral cortex and hippocampus, respectively.
Results indicate that endogenous Na+, K+-ATPase inhibitor
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.
endobain E differentially modifies the expression of
NMDA receptor subunits.