ADIPOCYTES IN BREAST CANCER: LIPOLYTIC AND MITOCHONDRIAL ALTERATIONS IN THE TUMOR MICROENVIRONMENT

MARIANA GANTOV; NATALIA SANTINO; ALICIA RITA AMATO; CECILIA LOTUFO; MARÍA LUJÁN CROSBIE; CELESTE FRASCAROLLI; JUDITH TONEATTO; PRISCILA PAGNOTTA; RUBÉN DRESZMAN; ANABELA URSINO; JUAN CARLOS CALVO

Congreso; REUNIÓN ANUAL DE SOCIEDADES BIOCIENTÍFICAS SAIC . SAI . SAFIS; 2020

Breast cancer cells induce metabolic reprogramming of peritumoral adipocytes, promoting adipocyte delipidation and atrophy/regression. We have previously reported that peritumoral adipocytes showed a decrease in the expression of Plin1, which together with HSL and ATGL regulate the lipolytic process, and smaller lipid droplet size. In this study we analyzed the effect of breast cancer cells on expression of several proteins involved in the lipolytic process in explants of human adipose tissue of tumor (hATT) and normal (hATN) breasts. Also, we evaluated the effect of conditioned media (CMs) from hATT and hATN on the lipolytic process of 3T3-L1 adipocytes. The expression of ATGL and HSL was analyzed by immunohistochemistry and Western blot. Levels of both proteins were increased in hATT with respect to hATN (p