MAMMARY GLAND-SPECIFIC REGULATION OF GNRH AND GNRH-RECEPTOR GENE EXPRESSION IS LIKELY PART OF A LOCAL AUTOREGULATORY SYSTEM IN FEMALE VIZCACHAS (CHINCHILLIDAE: RODENTIA).

CORTASA, SANTIAGO A.; FIDEL, VICTORIA; FERNANDEZ, MARINA O.; HALPERIN, JULIA; CORSO, MARÍA C.; SCHMIDT, ALEJANDRO R.; CWIRENBAUM, RUTH; VITULLO, ALFREDO D.; PROIETTO, SOFÍA; FEEHAN, KEVIN; INSERRA, PABLO I.F.; DORFMAN, VERÓNICA B.

Mar del Plata

Congreso; Reunion conjunta de Sociedades de Biociencia; 2019

SAIC

Our laboratory has recently reported gonadotropin-releasing hormone (GnRH) protein expression in mammary gland (MG) epithelial cells of vizcachas during pregnancy and lactation. In addition, we also shown that prolactin (PRL) modulates GnRH MG content. The present work aims to study GnRH gene expression in MG and also, to explore a possible GnRH-autocrine mechanism over this tissue. We amplified GnRH, GnRH-receptor (GnRH-R), early growth response factor 1 (Egr-1), PRL, PRL-receptor (PRL-R) and α-lactalbumin by RT-PCR using mRNA from MG at different stages of pregnancy and lactation. We established local transcription of GnRH at all the analyzed stages and the amplicon sequencing confirmed GnRH identity. Maximum transcription of GnRH occurred at early pregnancy and this coincides with maximum transcription of GnRH-R (p