Homeostasis loss in VIP deficient mice is associated with altered regulatory T cells recruitment

GALLINO LUCIA; E.BERENSZTEIN, ; PEREZ LEIROS C; N.SARACO; PARBORELL F; V. HAUK; PASCUALI N ; RAMHORST, ROSANNA

Congreso; International Federation of Placenta Associations 2019 (IFPA2019) junto a 8th Latin American Symposium on Maternal-Fetal Interaction and Placenta (VIII SLIMP),; 2019

International Federation of Placenta Associations 2019 (IFPA2019)

Objetive: In order to have a successful pregnancy, it is required a balance between proinflammatory and tolerogenic mediators. Since VIP (vasoactive intestinal peptide) is a master regulator associated with Tregs induction and recruitment, we aim to understand the relevance of VIP in normal pregnancy with focus on the Tregs. Methods: We used two mice models: VIP Knockout and Foxp3-IRES-GFP, both in a C57 background. The serum and tissues obtained were analyzed by flow cytometry, RT-PCR and histological staining. We also performed Foxp3-adoptive cell transfer and migration assays.Results: On the day of estrus we found histological differences between the ovaries and uterus of a WT mice vs VIP knockout mice (-/-). In uterus, these changes were accompanied with undetectable levels of expression Foxp3 in the (-/-) group, higher expression of RORγt and a decrease in IL-10 (p