El oligonucleótido IMT504 no revierte la diabetes inducida por dieta restringida en proteínas pero altera el peso corporal en machos

BIANCHI MS; DURST M; HERNANDO-INSÚA A; MONTANER A; ARANY E; LIBERTUN C; LUX-LANTOS V

Ciudad Autónoma de Buenos Aires, Argentina

Jornada; XI Jornada de la Sociedad Argentina de Biología; 2009

Sociedad Argentina de Biología

We have previously shown that the oligonucleotide IMT504 improves streptozotocin-induced diabetes in rats, in addition of having an anorexigenic effect. Here we studied the effect of IMT504 on diabetes induced in rats by low protein diet given to dams during pregnancy and lactation. Wistar rats were fed with an 8% protein diet (LP) or control diet (C: 20%) during pregnancy and lactation. Starting on the day of weaning (day 22) pups were injected daily with IMT504 (4 mg/0.2 ml saline) for 10 days (BP-IMT504 and C-IMT504) or with saline (BP-SAL y C-SAL). Body weight (BW) and glycemia evolution were studied. Glucose and insulin tolerance tests were performed in adulthood. Blood glucose was similar in all groups.  BWs in LP females were lower than in C females, without differences due to IMT504. In males, LP diet induced a reduction in BW which was aggravated by IMT504 treatment [BW at 60 days (g): C-SAL: 348.1±7.55 (3), C-IMT504: 349.6±13.82 (4), BP-SAL: 282.7±8.55 (4), BP-IMT504: 243.8±8.46 (4), two way-ANOVA: interaction: p<0.05, BP-IMT504 different from all: p<0.01]. Although IMT504 did not improve the diabetic condition in this model of diabetes, it had a clear effect on BW in males. (CONICET-ANPCYT-UBA)