CONICET | Buscador de Institutos y Recursos Humanos

Regulatory actions of melatonin in rodent Sertoli cellsSoledad Paola Rossi1,2; María Eugenia Matzkin1,2; Eliana Herminia Pellizzari3, Silvina Beatriz Meroni3, Ricardo Saúl Calandra 1; Artur Mayerhofer 4; Mónica Beatriz Frungieri1,5.1_Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428, Ciudad de Buenos Aires, Argentina2_Departamento de Bioquímica Humana, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Ciudad de Buenos Aires, Argentina3_Centro de Investigaciones Endocrinológicas, CONICET, Hospital de Niños R. Gutiérrez, Gallo 1330 (1425), Ciudad de Buenos Aires, Argentina4_BioMedizinisches Centrum (BMC), Ludwig-Maximilians-University (LMU), Cell Biology, Anatomy III, Großhaderner Str. 9, D-82152, Planegg, Germany.5¬_Cátedra de Química, Ciclo Básico Común, Universidad de Buenos Aires.Melatonin, Oxidative stress, lactate dehydrogenase, Sertoli cellsMelatonin acting through the hypothalamus and pituitary regulates testicular function. In addition, direct actions of melatonin at testicular levels have also been detected. Recently, we described a protective role of melatonin against free radical damage in testicular mast cells. The aim of this study was to investigate the participation of melatonin in the regulation of the oxidative state and lactate dehydrogenase (Ldh) expression in Sertoli cells. For this purpose, we used Sertoli cells isolated from 21 days old Syrian hamsters and the murine TM4 Sertoli cell line, both expressing the melatonergic receptor subtype 1.After 120 min incubation in the presence of the oxidizing agent tert-butyl hydroperoxide (TBHP; 500µM), ROS production from TM4 cells was significantly increased. This action was prevented by 1M melatonin (flurometric assay, arbitrary units, a.u.: control: 6761 ± 501a; TBHP: 13084 ± 686b; melatonin + TBHP: 9085 ± 326c; X ± SEM;P