MELATONIN AMELIORATES CHEMOTHERAPY-INDUCED OVARIAN DAMAGE

NATALIA PASCUALI; DALHIA ABRAMOVICH; OUBIÑA GONZALO; YAMILA HERRERO; LEOPODINA SCOTTI; FERNANDA PARBORELL

Congreso; REUNIÓN ANUAL de Sociedades de Biociencia 2019 SAIC . SAFE . SAB . SAP; 2019

SAIC . SAFE . SAB . SAP

Melatonin (MEL) is a lipofilic molecule which can act as a potent reactive oxigen species scavenger. MEL has been shown to protect tissues from severe oxidative stress. Premature Ovarian Failure (POF) is characterized by the depletion of ovarian follicles in young women, which may be caused by chemotherapy. Current treatments for POF, mainly hormone therapies, are not completely effective. One of the mechanisms underlying chemotherapy-induced POF is a strong increase in oxidative stress, which results in an aged ovarian phenotype. The present study proposes the application of MEL as a strategy to protect the ovary in patients undergoing chemotherapy.To induce POF, cyclophosphamide (CTX, 75mg/kg, i.p.) was applied in F1 mice (C57XBalbC 8 weeks old) on day 1. MEL (15mg/kg, i.p.) was applied on days 1, 6 and 11. Sacrifices were made at day 15. The ovaries were isolated for histological analysis and protein extraction for Western Blot assays. For all data analysis ANOVA followed by Tukey test were performed. An ovarian morphological analysis showed that CTX increased the % of primary and atretic follicles and reduced the % of antral follicles (p