Pitiutary release of lh is modulated by prl in a time exposure-dependent manner.

CORTASA SANTIAGO ANDRÉS, PROIETTO S, SCHMIDT AR, CORSO MC, INSERRA PIF, DI GRIORGIO NP, LUX-LANTOS V, VITULLO AD, DORFMAN VB, HALPERIN J.

Mar del Plata

Congreso; LXIII Reunión Científica Anual. Sociedad Argentina de Investigación Clínica (SAIC); 2018

SAIC

The progressive decline of circulating progesterone (P4) during the first half of gestation elicits surges og GnRH and LH, followed by an ovulatory event that leads to the development of numerous accessory corpora lutea (CL). The naturally calcified and steroidogenically poor placenta strongly points to CL as the main source of P4 during pregnancy. For other rodents, an essential role of prolactin (PRL) in CL maintenance and P4 production has been clearly established. To investigate PRL relevance over the hypothalamus-hypophysis-ovarian axis performance, we analyzed PRL effect over LH release in vizcachas under two experimental conditions: 1) LH release of pituitary after one week in vivo treatment with Sulpiride 20mg/kg i.m. twice a day (PRL Chronic treatment, CRtx); 2) 6 h-culture of pituitary explants with serum of animals before/after CRtrxt animals (PRL Acute treatment, ACtx). Success of Sulpiride treatment in inducing a hyperprolactinemic condition was corroborated by the increase of both PRL transcription and immunoexpression compared to that of control pituitaries (p