Low Level Laser Therapy (LLLT) reduces chemotherapy-induced ovarian damage

SCOTTI, LEOPOLDINA; HIGUERA, JAVIER; OUBIÑA G; PASCUALI, NATALIA; NEGRI MONTES, LUCRECIA; VELÁZQUEZ, CANDELA; ABRAMOVICH, DALHIA; PARBORELL, FERNANDA

Buenos Aires

Congreso; XX Jornadas Anuales de la Sociedad Argentina de Biología (SAB) junto a la Sociedad Uruguaya de Biociencias (SUB); 2018

Sociedad Argentina de Biología

It is known that LLLT has beneficial effects on several processes including wound healing, pain and inflammation. LLLT modulates biological functions, including cell proliferation, apoptosis and angiogenesis, which in the ovary are intimately related to fertility. Premature Ovarian Failure (POF) is characterized by the disappearance of ovarian follicles in young women, which may be caused by chemotherapy. Current treatments for POF, mainly hormone therapies, are not completely effective. The present study proposes photobiomodulation as a strategy to protect the ovary in patients undergoing chemotherapy. The objective was to analyze the effect of LLLT in a) a chemotherapy-induced POF model in adult mice and in b) rat granulosa cell culture exposed to chemotherapy. For a), POF was induced with cyclophosphamide (CTX, 75mg/weight) in F1 mice (8 weeks) and LLLT (8, 12 and 16 Joule) was applied. For b), a rat primary granulosa cell culture in the presence of doxorrubicin (DX, 10uM) was performed and LLLT (8, 12 and 16 J) was applied. To characterize ovarian morphology of our experimental groups, a quantification of follicular structures was performed. CTX increased the % of atretic follicles and diminished the % of primary and preantral follicles compared to control group (p