IMPACT OF IMT504 ON THE IMMUNE SYSTEM OF FEMALE NON-OBESE DIABETIC (NOD/Ltj) MICE

STEFANIA BIANCHI; MARTA TOSCANO; VERÓNICA MARTINEZ ALLO; VICTORIA ADELA LUX-LANTOS; CARLOS LIBERTUN; MARÍA SILVIA BIANCHI

Colorado

Congreso; 19th Annual Colorado Immunology Conference; 2018

Department of Immunology and Microbiology of the University of Colorado School of Medicine

Immunomodulatory oligonucleotide IMT504 (IMT) improved glycemia, beta cell function, and reduced leukocyte islet infiltration in spontaneous autoimmune diabetic mice (female NOD/Ltj). We analyzed the effects of IMT on immune parameters. Female diabetic NOD/LtJ mice (two consecutive glycemia levels (Gly) ≥ 250 mg/dl) were treated daily with IMT for five days (20mg/kg/day) or saline as control (DC). The day following the last injection, fasted Gly was measured and mice were sacrificed. Pancreatic leukocytes and splenocytes were obtained, immune cell populations (by flow cytometry) and cytokines expression (by q PCR) were analyzed respectively. DC mice showed 11% (2/19) spontaneous reversion of the diabetic condition whereas IMT treatment improved Gly in 72% of mice (13/18) (χ2: DC vs IMT20: p