Metabolic Syndrome in female mouse: effect on fertility and ovarian angiogenesis

NEGRI MONTES L; DALTON N; ABRAMOVICH D; VELAZQUE C; L SCOTTI; F. PARBORELL; OUBIÑA G,; DI SIERVI A

Congreso; LXIII Reunión Científica Anual de Investigaciones Clínicas (SAIC); 2018

SAIC

Metabolic syndrome (MS) is a cluster of conditions that comprises at least three of these features: overweight, hypertension, hypertriglyceridemia, hyperglycemia and low HDL. Metformin (MET) is a hypoglycemic drug used for type 2 diabetes. MET is capable of improving fertility in these women.Objective: To evaluate oocyte quality, in vitro fertilization, folicular development, and ovarian Platelet-Derived Growth Factor B (PDGFB) in a MS female mouse model. To study ovarian effects of MET.Methods: C57BL/6 female mice, four weeks old, were fed with a high fat diet (HFD) during 17 weeks. Control animals received standard diet (SD). A group of HFD also received MET. The estrous cycle and glycemia was evaluated. The animals were sacrificed and serum was collected to measure cholesterol and triglycerides. One ovarywas used to obtain proteins and the other for histological studies. Another set of animals was superovulated with eCG and hCG. Animals were sacrificed and the number of ovulated oocytes counted. By an in vitro fertilization we measured the percentage of fertilized oocytes and calculated the percentage of these oocytes that developed to blastocyst stage.Results: HFD group showed higher weight, cholesterol and triglyceridemia. Not differences were found in the number of ovulated or fertilized oocytes. The percentage of embryos that developed to blastocyst was lower in the HFD group. MET reversed this effect. The HFD group spent more time in diestrous and less time in estrous and theMET group spent more time in metaestrous and less time in estrous compared to HFD group. Also, MET raised ovarian PDGFB. Finally, follicular dynamics was altered in HFD animals and MET improved it, raising the percentage of corpora lutea and reducing the percentage of atretic follicles.Conclusions: MS alters follicular dynamics and oocyte quality. MET administration would have a beneficial effect on fertility in MS.