EFFECT OF BENZOPHENONES 2 AND 3 ON INSULIN SECRETION REGULATED BY AUTOPHAGY MODULATORS IN PANCREATIC BETA CELL LINE MIN6B1

SZULAK FLORENCIA; SORIANELLO ELEONORA; FERNÁNDEZ MARINA OLGA; BECÚ-VILLALOBOS DAMASIA

CABA

Jornada; XX JORNADAS ANUALES DE LA SOCIEDAD ARGENTINA DE BIOLOGÍA (SAB), XVII JORNADAS DE LA SOCIEDAD URUGUAYA DE BIOCIENCIAS (SUB), Segundas Jornadas Rioplatenses de Biología; 2018

Sociedad Argentina de Biología

Benzophenones are present in cosmetics, plastics and other industrial goods to protect human skin or products against direct exposure to ultraviolet (UV) radiation. Benzophenone-2 (BP2) and oxybenzone (benzophenone-3 or BP3) are common ingredients in sunscreen and have proven to be endocrine disruptors. Autophagy is an evolutionary conserved cellular self-degrading mechanism. It involves degradation and recycling of cellular components and plays an important role in cell homeostasis. Recent data reveal that benzophenones alter the autophagy process. The aim of the present study is to evaluate the effect of BP2 and BP3 on pancreatic beta cell function, more precisely, on insulin (INS) secretion in the presence of autophagy modulators. For this purpose a murine pancreatic beta cell line MIN6B1 was cultured in the presence of 10-5 M BP2 or BP3, or DMSO as a control, during 24h. In order to modulate autophagy, some groups were also incubated with Rapamycin (RAPA, autophagy inductor) or Chloroquine (CQ, autophagy flux inhibitor), alone or in combination with BP2 and BP3. Conditioned media were collected, centrifuged and cleared supernatants were subjected to a radioimmunoassay (RIA) for insulin determination. Our results show that BP2 alone did not change INS secretion in MIN6B1 beta cell line in comparison to the control condition while BP3 significantly inhibited INS secretion (p