Bioactive sphingolipid Ceramide-1-phosphate (C1P) protects against age-related ovarian dysfuntion/decline in female mice

ABRAMOVICH D; DI PIETRO, M.; PARBORELL F; OUBIÑA G,; L SCOTTI; COHEN D; N PASCUALI

Rome

Congreso; 17 th World Congress of the Academy of Human Reproduction; 2017

17 th World Congress of the Academy of Human Reproduction

For a variety of reasons, many women postpone childbearing over 38 years, and a considerable proportion of aged female become infertile. Ovarian aging is dominated by a progressive loss of primordial follicles and decline in the quality of oocytes. The activation of ovarian angiogenesis has emerged as a new strategy for the improvement of age-related decline of ovarian response. Since the bioactive sphingolipid C1P is an important proangiogenic and anti-apoptotic factor, our aim was to study whether C1P can improve the ovarian response and angiogenesis in aged female mice.Aged female mice (26-31 weeks) received C1P (10µl/ovary; 50µM) under the bursa of one ovary and vehicle on the other ovary, and were sacrificed 48h post-surgery. Young mice (6-9 weeks) were used as control. Ovaries were isolated for histological and immunohistochemical analysis (VW and SMA; endothelial and periendothelial cell markers, respectively), radioimmunoassay (E2 and P4 concentrations) and western blot (pFoxo3a/Foxo3a and AMH; ovarian reserve markers).The % of primary (PF), preantral (PrF) and antral follicles (AF) in aged mice were lower than in young mice (p