STEROID-RELEASING NANOSTRUCTURED BIOMATERIAL COATING FOR CONTROLLING CELL PROLIFERATION AND HORMONE SECRETION

PAOLO CATALANO; VICTORIA LUX-LANTOS; MARTIN BELLINO; PAULA AROCENA; MARINA FERNANDEZ; NATALIA SCILLETTA

CABA

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

The development of functional biomaterials at the frontiers of cell biology, chemistry and materials sciences is of great research interest. The possibility of organizing matter in the nanoscale has great relevance in designing biomaterials for the regulation of specific cell functions. This could be achieved through molecules loaded within the material that can be delivered to cells with high efficiency. In this context, the aim of this work was to develop new biomaterials using titanium dioxide mesostructured thin films containing biocompatible reservoirs for storage and release of steroid hormones, capable of modifying cells behavior. These films were synthesized on glass slide pieces by sol-gel and molecular self-assembly methodology to facilitate the inclusion of estradiol within the template micelles during film preparation. The release of loaded hormone to culture media was evaluated in vitro by RIA. Biocompatibility and functionality of as prepared film coatings was assessed by culturing GH3 cells on them for 48 h and by evaluating the effects of the released steroid on cell proliferation and PRL secretion. Estradiol release from film coatings did not depend on the amount of loaded hormone assayed, showing a progressive pattern and reaching 500 nM at 48 h. Proliferation of GH3 cells and PRL secretion was stimulated (Relative proliferation index: 1.37 vs 0.98; PRL: 4.46 µg/mL vs. 3.52 µg/mL; p