Autologous seminal plasma prevents sperm capacitation of equine sperm

NEILD D; MIRAGAYA M; FERRANTE A; CALDEVILLA M; VAZQUEZ LEVIN, M; MARIN BRIGGILER, CI; CARRETERO MI

Buenos Aires

Congreso; XVIII Jornadas Anuales Multidisciplinarias de la Sociedad Argentina de Biología; 2016

Sociedad Argentina de Biología

The seminal plasma (SP) has been reported to modulate sperm function(s) in several species. The aim of this study was to evaluate the effect of autologous SP on stallion sperm in vitro capacitation. Semen from fertile stallions (n=4; 3 replicates each) was subjected to Androcoll-ETM colloid centrifugation. Selected sperm were incubated under capacitating conditions for 3 h in MW-BSA medium alone or with 2.5, 5 and 10% SP. Sperm capacitation was evaluated by a) chlortetracycline (CTC) staining (B-pattern), b) immunocytochemical detection of tyrosine phosphorylated proteins (PTyrP) following HOST (HOST/PTyrP) (correlation between CTC-B pattern and HOST+/PTyrP+, r=0.81; p