NITRIC OXIDE SYNTHASE (NOS) EXPRESSION IN THE HIPPOCAMPUS FROM THE PROGENY OF EXPERIMENTAL DIABETES MOTHERS. EFFECT OF THE INTAKE OF VEGETABLE OILS

MAGRINI-HUAMAN, R.N.; DELGADO, J.F.; TAPIA, A.; REY, M.; VEGA, M.C.; FERESIN, G.E.; COIRINI, E.; INGHANI, M.; COIRINI, H.

Ciudad Autonoma de Buenos Aires

Congreso; XVIII Jornadas Anuales Multidisciplinarias de la Sociedad Argentina de Biología; 2016

Sociedad Argentina de Biologia

Uncontrolledmaternal hyperglycemia during pregnancy produces alterations in the developingnervous system of the offspring. In previous studies, we showed that pistachiooil (PS) increases exploratory activity in the open field test. The aim of thisstudy was to: 1) Evaluate the expression of hippocampal NOS in adult rats (SD,male/female, 8 months old) born from control mothers (CO) or mothers withexperimental diabetes (streptozotocin, 30 mg /Kg iv; DO) and 2) Study theeffect of early dietary supplementation with corn oil (MZ), extra virgin oliveoil (OL) and PS. The oils were administered from day 2 to 62 of age (8μl /15g).NOS expression was analyzed by western blot. In males NOS level wassignificantly higher in the DO than in CO (ANOVA, p <0.001) with or withoutoil supplementation. CO highest expression was observed with PS (p <0.001),whereas in DO it was found with OL (p <0.01), no significant differencesbetween CO-PS and DO-PS (p = 0.871) was found. The results were similar infemales, higher level of NOS in DO than CO (p <0.001) and OL and PSincreased NOS expression in CO and DO (p <0.01) respectively. These studiesindicate that early supplementation with oils rich in ω3 to DO does notdecrease the hippocampal expression of NOS. In addition, it seems to be somesex dependent responses to the treatments. (CONICET-PIP0243;  PICTO/UCCuyo2009-0158;  CICITCA UNSJ-IDeA1400.0107/2012).