LXR EXPRESSION CHANGES IN LIVER (L) AND HYPHOTALAMUS (HT) BY A HIGH FAT DIET

REY, M.; TAPIA, A.; FERESIN, G. E,; COIRINI, H.

Chascomus

Congreso; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014

Sociedad Argentina de Biologia

In previous assays, we evaluated the effect of different high fat diets on liver X receptors (LXR), involved in reverse cholesterol transport, in rat L and HT. In this work, the effect of a diet, with the addition of bovine fat (38%) + cholesterol (2%) + cholic acid (0.2%; FCC), and its subsequent return to normal diet (C) were analyzed. Male rats of 50 days of age, were fed with FCC diet for 10 days, and then turned to C diet the following 7 days. Assays were performed at 8, 10, 12 and 17 days. By colorimetric methods, changes respect control animals (fed only with C) were observed in serum cholesterol levels (228%, 209%; 63%; 45%) and triglycerides (8d=56%; 10d=85%; p<0.05). With WB, LXRa significant increases were observed in L at 10d=6% and HT at 12d= 4% (p<0.05). In the other hand, LXRb increases were observed in both tissues (6-15% and 17-45%; p<0.05) in all days studied. Relation between subtypes (a and b) expression, could be directly involved in cholesterol regulation mechanisms in both tissues (CONICET-PIP860).