Alpha2 and Beta-adrenergic action on human breast cancer cells growing in nude mice.

PEREZ PIÑERO CECILIA; ENTSCHLADEN FRANK; LUTHY ISABEL A.

Washington, DC

Congreso; 101st Annual Meeting 2010 - American Association for Cancer Research (AACR); 2010

American Association for Cancer Research

Breast cancer, the most common cancer amid women in the majority of countries, is among the most stressful of diseases. The principal effectors of the stress system include the catecholamines epinephrine and norepinephrine. The catecholamines bind to alpha1-, alpha2- and beta-adrenoceptors (AR). Our group has recently described alpha2-adrenoceptors in human tumor and non-tumor breast cell lines by reverse transcription (RT)-PCR, immunocytochemistry and binding assays. Moreover, the stimulation by alpha2-adrenoceptor agonists was associated with increased cell proliferation and tumor growth. On the other hand, we observed a reduction in tumor growth when the animals are treated with the pharmacological alpha2-antagonist RAUWOLSCINE (RAW). The expression of beta2-AR has been described in different experi¬men¬tal models for breast cancer. The aims of the present work were: to assess the effect of beta-adrenergic compounds in the human breast cancer cell lines growing in nude mice (IBH-4 and IBH-6); to study the migration potential of these cell lines using video microscopy in three dimensional collagen lattices knowing that migration of tumor cells is a prerequisite for invasion and metastasis development. The daily administration of 1,2mg/kg of the SALBUTAMOL (SALB) significantly diminished tumor growth. The same result was seen when using the alpha2-antagonist RAW (0,5mg/kg). As an example for tumor IBH-4 treated with SALB, on day 20: 444.16  76.59 mm3 vs. 843.40  189.41, p