Endometrial physiology and early pregnancy are affected in a mouse model of metabolic syndrome.

CARNOVALE N; FRUNGIERI M; PALAZZI J; MERESMAN G; VELAZQUEZ C; MATZKIN ME; ABRAMOVICH D; STELLA I; FRITZLER A; BILOTAS M

Congreso; LXVII reunión anual de la sociedad argentina de investigación clínica (SAIC); 2022

The prevalence of metabolic syndrome (MS) is rising globally. There is growing evidence which links MS to poor reproductive health. The aim of this study was to investigate the effect of MS on endometrial physiology and early pregnancy in mice treated or not with metformin (Met). Sixty 21-day-old C57BL/6 female mice were used: 20 were fed with a control diet (C) and 40 with a high-fat diet (HFD). At week 12, 20 HFD animals started treatment with Met 300 mg/kg in drinking water (HFDMet). At week 16, 10 mice for each group were sacrificed. The rest of the animals were mated with proven fertility male mice and sacrificed on day 7 of pregnancy. This mouse model of MS was previously characterized showing augmented levels of cholesterol, triglycerides, HDL, glycemia and insulin, and increased visceral and gonadal adipose tissue.Non-pregnant HFD mouse endometrium showed an increase in: epithelial apoptosis evaluated by TUNEL (p