IBYME 02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THIOL REDUCTION AND PROTAMINE EXCHANGE: SIMULTANEOUS EVENTS DURING HUMAN SPERM DECONDENSATION?
Lugar:
Buenos Aires
Reunión:
Jornada; XII Jornadas Multidisciplinarias de la Sociedad Argentina de Biología; 2010
Institución organizadora:
Sociedad Argentina de Biologia
Resumen:
Previous results indicate that heparin (Hep) cooperates with glutathione(GSH) in protamine thiol reduction during human spermdecondensation. The aim of this study was to evaluate the effect ofHep on chromantin thioreduced status using a direct methodology.Isolated nuclei from normospermic donors (OMS) were incubated(37°C) with GSH (10mM) or DTT (0.1mM) (30) and Hep (46uM)(0, 5, 15, 30). Chromatin thioreduced status was evaluated by fluorescencemicroscopy with thiol reagent monobromobimane andquantified (arbitrary units) using ImageJ. DTT was a more potentthiol reducing agent than GSH (98±2 vs. 62±2, ANOVA-Dunn,p<0,01). Thiol reduced status increased significantly after 5 exposureto Hep with GSH as thiol reducing agent (62±2 vs. 102±3,ANOVA-Dunn, p<0.001) but not with DTT. These results confirmthat Hep not only behaves as protamine acceptor but also cooperatesin chromatin thiol reduction by GSH, suggesting that both processestake place simultaneously.
