IBYME 02675
INSTITUTO DE BIOLOGIA Y MEDICINA EXPERIMENTAL
Unidad Ejecutora - UE
artículos
Título:
Sub-ångström cryo-EM structure of a prion protofibril reveals a polar clasp
Autor/es:
RODRIGUEZ, JOSE A.; CASCIO, DUILIO; HELGUERA, GUSTAVO F.; NOVIKOVA, IRINA V.; HERNANDEZ, EVELYN; GLYNN, CALINA; GONEN, TAMIR; SAWAYA, MICHAEL R.; MCFARLANE, HEATHER T.; ZEE, CHIH-TE; MARTYNOWYCZ, MICHAEL W.; GALLAGHER-JONES, MARCUS; EISENBERG, DAVID S.; EVANS, JAMES E.; GOLDSCHMIDT, LUKASZ; MIAO, JENNIFER; BOYER, DAVID R.
Revista:
Nature Structural & Molecular Biology
Editorial:
Nature Publishing Group
Referencias:
Año: 2018 vol. 25 p. 131 - 134
ISSN:
1545-9993
Resumen:
The atomic structure of the infectious, protease-resistant,β-sheet-rich and fibrillar mammalian prion remains unknown.Through the cryo-EM method MicroED, we reveal the subångström-resolution structure of a protofibril formed by awild-type segment from the β2?α2 loop of the bank vole prionprotein. The structure of this protofibril reveals a stabilizingnetwork of hydrogen bonds that link polar zippers within asheet, producing motifs we have named ?polar clasps?.
