INVESTIGADORES
OLIVERA nelda Lila
artículos
Título:
Lactic acid bacteria isolated from fish gut produce conjugated linoleic acid without the addition of exogenous substrate
Autor/es:
MARIA SOLEDAD VELA GUROVIC; ALEJANDRO GENTILI; NELDA LILA OLIVERA; SUSANA RODRIGUEZ
Revista:
PROCESS BIOCHEMISTRY - (Print)
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2014 vol. 49 p. 1071 - 1071
ISSN:
1359-5113
Resumen:
The production of conjugated linoleic acid (CLA) by four strains of lactic acid  bacteria isolated from fish, i.e., Leuconostoc mesenteroides H20, Leuconostoc  mesenteroides H22, Leuconostoc lactis H24 and Lactobacillus pentosus H16, was  evaluated in MRS broth and on MRS agar. The bioconversion and production of CLA  by resting cells were also assessed. Linoleic acid was detected in cultures grown on  agar at percentages of up to 18.3 % (w/w) of total fatty acid, and conjugated isomers  were found in the fatty acid profiles of Lactobacillus pentosus H16. The percentage of  CLA relative to total fatty acid increased from 5.68 ± 1.65 % to 23.69 ± 0.79 % when  resting cells were removed from agar plates and incubated without the addition of  exogenous linoleic acid as a substrate. When Lactobacillus pentosus H16 cells were  incubated with linoleic acid, cyclization and changes in monounsaturated fatty acid  percentages were observed instead of conjugation. These results show that growth  on a solid support is required for CLA production. More significantly, an increase in  the CLA content could be achieved by incubating resting cells without exogenous  substrate.