INVESTIGADORES
MORILLA Maria Jose
artículos
Título:
Liposomal benznidazole: a high-performance liquid chromatographic determination for biodistribution studies.
Autor/es:
MORILLA MJ1, BENAVIDEZ PE, LOPEZ MO, ROMERO EL.
Revista:
JOURNAL OF CHROMATOGRAPHIC SCIENCE
Editorial:
PRESTON PUBL INC
Referencias:
Año: 2003 vol. 41 p. 405 - 409
ISSN:
0021-9665
Resumen:
In this work, an isocratic high-performance liquid chromatographic
method for quantitation of liposomal benznidazole (BNZ) in biological
tissues is presented. The method comprises protein precipitation
together with an efficient extraction of bulk or liposomal BNZ with
acetonitrile-dimethylsulfoxide (1:1, v/v) at a 2:1 (extraction
solvent-tissue matrix, v/v or /vw) ratio; the process is completed by a
final precipitation with trichloroacetic acid. The resultant
supernatants are assayed chromatographically using a Kromasil C18 (25- x
0.4-cm i.d., 100 A, 5- microm particle size), with an isocratic mobil
phase consisting of acetonitrile-water (40:60, v/v), a flow rate of 0.9
mL/min, and detected at 324 nm. Bulk BNZ is used as a reference standard
for the analysis of samples containing liposomal BNZ. The assay is
linear over a concentration range of 0.75 (the lowest quantity of
analyte determined with precision and accuracy of >or= 20%) to 25
microg/mL-g in all liquid and solid matrices. Within-day precision is
better than 6.4% in plasma and 8.6% in liver, the same for the two
assayed concentrations. Between-day precision is 5.4% and 12.3% in
plasma and 9% and 6.9% in liver for the two assayed concentrations,
respectively. The absolute recoveries range between 70% and 97%.
Therefore, the method is accurate and precise to be employed for
detection of minor quantities of liposomal BNZ in biological tissues.