PERSONAL DE APOYO
MONCADA melisa
congresos y reuniones científicas
Título:
Arachidonic acid modulation of BK (Slo1) channels: Role of the β1 accessory subunit
Autor/es:
MONCADA MELISA; DUCCA GERÓNIMO; ORSI FEDERICO; MILESI VERÓNICA; MARTÍN PEDRO
Lugar:
La Plata
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Biofísica (SAB).; 2018
Institución organizadora:
Sociedad Argentina de Biofísica (SAB)
Resumen:
Arachidonic acid (AA) is a polyunsaturated fatty acid involved in modulation of several ion channels activity. Previously, we reported that 10 µM AA activates the high conductance Ca2+- and voltage-dependent K+ channel (BK) in human vascular smooth muscle cells where the α subunit of BK is expressed together with the accessory β1-subunit (β1) [1]. In this work, we studied in depth the action mechanism of AA using the patch-clamp technique on BK channel heterologously expressed with β1. 10 µM AA activated BK only in presence of β1, changing the voltage dependence of activation (left shift on G-V curve, ΔV1/2= -55.2 mV ± 4,4; n=3; p
