ISOLATION AND CHARACTERIZATION OF GLYPHOSATE DEGRADING

MASSOT, F; OSTERA, JM; VAZQUEZ, S; BAILLARES, M; GIULIETTI, AM; MERINI, LJ

Mar del Plata

Congreso; 51 Annual Meeting - Argentine Society for Biochemistry and Molecular Biology; 2015

SAIB

The Humid Pampa is the main agricultural region in Argentina and one of the most important crop fields in SouthAmerica, covering about 52 million ha. As part of the modern agriculture practices, more than 300,000 tons ofpesticides are applied every year, were about 65% are formulations of the herbicide glyphosate (N?phosphonomethylglicine). To develop an efficient, eco?friendly, solar driven system to mitigate the environmentalimpact of glyphosate use in the agricultural exploitation context is a priority strategy. To achieve this goal, the aim ofthis work is to find and characterize glyphosate degrading bacteria associated to Lotus spp. roots. This plant?bacteriasystem will be use to enhance the glyphosate degradation in the rhizosphere influence zone and reduce its residues insoils and running off waters. To carry out this objective, bulk and Lotus rhizospheric soil samples were collectedfrom both, glyphosate treated and control pasture plots. Batch liquid cultures were performed using basal salt mediaenrichment with glyphosate as sole carbon or phosphorus source respectively. Among isolates, two glyphosate?degrading strains were selected and culturally/genotipically characterized. Glyphosate degradation in culture mediawas determined by FMOC?Cl derivatization followed by UPLC. In addition, plant growth promotion capabilitieswere also determined for both strains.