INVESTIGADORES
MERINI Luciano Jose
informe técnico
Título:
Innovative approaches to understand complex microbial communities for
Autor/es:
GIULIETTI, AM; FLOCCO, CG; MERINI, LJ; CUADRADO, V
Fecha inicio/fin:
2002-11-01/2005-10-30
Naturaleza de la
Producción Tecnológica:
Biotecnología ambiental
Campo de Aplicación:
Rec.Nat.Renov.-Conservacion y preservacion
Descripción:
The primary objective of ACCESS has been to generate and establish the knowledge base for
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.
primary objective of ACCESS has been to generate and establish the knowledge base for
the eco-engineering of soil ecosystems polluted with herbicides, atrazine, 2,4-D, and their
derivatives. This was proposed to be achieved by 1) the detailed physical, chemical and
microbiological characterization of herbicide contaminated agricultural soils; 2) the design
and optimization of molecular methods to study changes in gene expression of the responses
to stress in microbial isolates and communities, the changes in diversity and catabolic
performance of complex microbial communities; 3) extension of the knowledge base of
microbial capabilities for degrading the target compounds and the influences on degradation
through community interactions; 4) evaluation, with newly developed tools and methods, of
the factors that positively affect biodegradation by microbial communities in microcosms of
increasing complexity, including plant-microbe interactions and 5) design of knowledgebased
farming practices to more actively reduce pollution caused by organochlorine
herbicides.
To reach the objectives, experimental areas in Chile and Argentina were chosen based upon
the applied agricultural practice. Monitoring tools were optimized with a special emphasis on
molecular biology diagnostics. DNA extraction procedures were adapted to the soil types
under study and a new RNA extraction method for agricultural soils rich in humic acids was
developed. Nucleic acid profiling and sequencing methods were optimized and applied for
rapid assessments of microbial community structure and function and a prototype phylogenybased
DNA array system was developed for identifying individual members of complex
communities. Catabolic gene profiling methods were developedand applied, to assess their
diversity under changing environmental conditions. Dedicated DNA arrays were developed
and successfully applied, to assess the effects of stress caused by substrate addition on the
general physiological status of soil bacteria. An in silico system was established to simulate
the degradation of any given compound through the global biodegradation network.
Advanced molecular diagnostic tools in combination with accurate analytic determinations
were applied, to evaluate the effects of environmental stress factors, specifically herbicides,
on microbial community composition and degradation performance in different experimental
systems ranging from pure cultures and consortia, soil microcosms, including plant
microcosms and field studies. New isolates capable of degrading the target herbicides were
obtained, specifically simazine degrading isolates were identified to be promising candidates
for bioaugmentation. Soil microcosms of different complexities were shown to be useful tools
for analyzing the degradation of herbicides, the effectiveness of bioaugmentation procedures,
the catabolic potential of microbial communities and the changes in bacterial communities
after herbicide exposure. Overall, the project developed a variety of new molecular tools
which focused on assessing community and catabolic gene structure and gene expression, as
well as linking biodegradation to host physiology. A multifaceted approach at the different
scales of complexity succeeded in generating a knowledge base which can be applicable for the
eco-engineering of sites polluted with atrazine, 2,4-D, and their derivatives.
Information on new molecular tools has been published or is in the process of being published.
The Meta-Router web page is available to the general public. All partners are available for
consultancy.