Simultaneous determination of saccharin and aspartame in commercial non-caloric sweeteners using the PLS-2 multivariate calibration method and validation by capillary electrophoresis

MIGUEL A. CANTARELLI; ROBERTO G. PELLERANO; EDUARDO J. MARCHEVSKY; JOSÉ M. CAMIÑA

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

American Chemical Society Publisher

Año: 2008 vol. 56 p. 9345 - 9349

0021-8561

A new method to determine a mixture for sweetener sodium saccharin and aspartame in commercial non-caloric sweeteners is proposed. A classical full factorial design for standards was used in the calibration step to build the partial least square (PLS-2) model. Instrumental data were obtained by means of UV-visible spectrophotometry. Salicylic acid was used as an internal standard in order to evaluate the adjustment of the real samples to the PLS model. The concentration of analytes in the commercial samples was evaluated using the obtained model by UV spectral data. The PLS-2 method was validated by capillary zone electrophoresis (CZE), finding in all cases, a relative error of less than 11% between the PLS-2 and the CZE methods. The proposed procedure was applied successfully to the determination of saccharin and aspartame in non-caloric commercial sweeteners.