A POLYMERIC NANOPARTICLE PLATFORM WITH TH1-DEPENDENT ADJUVANT PROPERTIES FOR IMMUNOMODULATION OF FOOD ALLERGY

RIZZO, GASTÓN; QUEREDA CORSO, MICAELA BELÉN; DOCENA, GUILLERMO; SMALDINI, PAOLA

San Luis

Congreso; Reunión Anual de la Sociedad Argentina de Inmunología 2023; 2023

Sociedad Argentina de Inmunología

Nanotechnology is increasingly assuming a significant role in the advancement of vaccine development. This technology can create diverse nanoparticles (Np) with adjustable composition, size, shape, and surface characteristics. Emerging advances in nanotechnology significantly impacted on biomedicine, vaccinology and health research. In this study, we analyzed polyallylamine (PAH) nanoparticles as a vehicle for antigen protection, delivery carrier, and adjuvant in formulating a mucosal therapeutic vaccine for food allergies.We studied the intracellular inflammasome activation on murine bone marrow-derived dendritic cells (BMDC) using Np and different inhibitors. Additionally, we stimulated bone marrow-derived macrophages (BMDM) from null mice for NLRP3, Caspase-1, Caspase-1/11, or gasdermin D (GSDM D) to identify the pathway involved. The secretion of IL-1β was used as a readout of cell activation. Np´s integrity and carrier capacity throughout the gastrointestinal tract and the adjuvant properties were assessed in a mouse model of IgE-dependent food allergy driven by cholera toxin and cow´s milk protein (CMP) as a food allergen. Sensitized mice were then treated by gavage of β-lactoglobulin encapsulated in Np. Clinical score and immunological parameters were assessed for evaluation of allergy suppression.We found that Np were up-taken by phagocytic cells and upon cell activation, IL-1β was secreted. Inhibitors of the inflammasome pathway abrogated the cytokine release (p