Peptides from Amaranth suppressed intestinal inflammation via NF-kB

QUEREDA, MICAELA; SMALDINI, PAOLA; RIZZO, GASTÓN; DOCENA, GUILLERMO

Virtual

Otro; IUIS-ALACI IMMUNO-COLOMBIA online Course"Mechanisms and Approaches to Immunotherapy for Cancer and Chronic Inflammatory Diseases"; 2021

IUIS-ALACI

Biological, nutritional and health benefits of amaranth have been highlighted in the last years.Proteins of amaranth exert anti-hypertensive, anti-oxidant, anti-thrombotic and anti-proliferativeeffects.The aim of this study was to analyze the anti-inflammatory effect of peptides from amaranth on NFkB intracellular pathway activation in intestinal epithelial cells, and in experimental intestinalinflammation, such as colitis and food allergy.Colon cell lines (Caco-2 and Caco-luc) were cultured with flagellin and amaranth peptides. CCL20-expression was evaluated by qPCR and NF-κB modulation was evaluation by light emission andqPCR, along with p65-nuclear translocation. In vivo studies included the oral administration of aformulation containing the peptide during the allergic sensitization or the colitis induction phase inBalb/c mice. Treatment efficacy was in vivo and in vitro evaluated.We found several peptides with anti-inflammatory capacity and we selected that with the highestability to suppress cell activation (decrease in CCL20 and light emission p