INVESTIGADORES
ORMAZABAL maximiliano Emanuel
congresos y reuniones científicas
Título:
Characterization of the key antigenic components of pertussis vaccine based on outer membrane vesicles
Autor/es:
ORMAZABAL MAXIMILIANO; BARTEL ERIKA; GAILLARD MARÍA EMILIA; BOTTERO DANIELA; ERREA AGUSTINA; ZURITA EUGENIA; MORENO GRISELDA; RUMBO MARTÍN; CASTUMA CELINA; FLORES DARÍO; MARTÍN MARIA JULIA; HOZBOR DANIELA
Lugar:
Philadelphia, PA
Reunión:
Congreso; 8th Vaccine & ISV Congress; 2014
Institución organizadora:
Elsevier - Vaccine journal
Resumen:
Pertussis has
resurged during the last two decades in different countries. In particular in
2010-2013 period large outbreaks were detected in US, Australia, UK and The
Netherlands with significant mortality in infants. The epidemiological
situation of pertussis point out the need to develop new vaccines and in this
sense we previously developed a new vaccine based on outer membrane vesicle
(OMV) which have been shown to be safe and to induce protection in mice. We
have here further investigated the properties of OMV vaccines; in particular we
investigated the contribution of pertussis toxin (PTx) and pertactin (Prn) in
OMVs-mediated protection against pertussis. A PTx-deficient OMVs and
Prn-deficient OMVs were obtained from defective B. pertussis mutants. The absence of PTx or Prn did compromise the
protective capacity of the OMVs formulated as Tdap vaccine. In vivo, whereas the protective efficacy
of the PTx-deficient OMVs in mice was comparable to Prn-deficient OMVs, the
protective capacity of both of them was significantly impaired when it was
compared with the wild type OMVs. Interestingly, using OMVs obtained from B. pertussis strain which does not
express any of the virulence factors but express the avirulent phenotype; we
observed that the protective ability of such OMVs was lower than that of OMVs
obtained from virulent B. pertussis
phase. However, it was surprising that although the protective capacity of
avirulent OMVs was lower, they were still protective in the used mice model.
These results allow us to hypothesize that OMVs from avirulent phase shares
protective components with all OMVs assayed. Using immune proteomic strategy we
identified some common components could play an important role in protection
against pertussis.