Application of the Ussing chamber technique to assess P-glycoprotein-mediated intestinal drug transport

BALLENT M.; LIFSCHITZ A.; VIRKEL G.; MATÉ L.; SALLOVITZ J.; LANUSSE C.

Leipzig, Alemania

Congreso; 11 th International Congress of the European Association for Veterinary Pharmacology and Toxicology (EAVPT); 2009

EAVPT

Transport proteins play an important role in the intestinal secretion of different drug molecules widely used in veterinary therapeutics. P-glycoprotein (P-gp) secrets a large number of endo-xenobiotic compounds from the intracellular to the extracellular domain by an ATP-dependent process (1).. Different in vitro models have been developed to predict P-gp activity. Caco-2 cell monolayers, derived from a human colonic adenocarcinoma, are widely used to estimate transepithelial passage for different P-gp drug substrates (2). The use of everted gut sacs has been proposed as a new in vitro model for quantification of the P-gp-mediated intestinal drug efflux (3, 4). However, new methodological tools are required to evaluate the physio-pharmacological features of the intestinal drug secretion process in ruminant species. The goal of the current work was to adapt the Ussing chamber technique to the assessment of the P-gp-mediated drug transport in rat and sheep intestine.