Flavonoids, Benzoic acids and Cinnamic acids isolated from shoots and roots of Italian rye grass (Lolium multiflorum Lam.) with and without endophyte association and arbuscular mycorrhizal fungus

PONCE M.A.; BOMPADRE M.J.; SCERVINO J.M; OCAMPO, J.A.; CHANETON E.,; GODEAS A.

BIOCHEMICAL SYSTEMATICS AND ECOLOGY

PERGAMON-ELSEVIER SCIENCE LTD

Año: 2009 p. 245 - 253

0305-1978

Phenolic compounds present in Lolium multiflorum Lam. were isolated and characterized.Significant differences in their distribution were found, in shoots and roots of plants with(Lmþ) and without (Lm) endophyte association, grown with (Micþ) and without (Mic)mycorrhizal fungi Glomus intraradices, indicating a systemic effect of these microorganismson the phenolics metabolism of L. multiflorum.We report the isolation of eight new flavonoids 7,8-dihydroxy-2-(4-hydroxyphenyl)-5-methoxy-4H-1-benzopyran-4-one (1), 3,7-dihydroxy-8-methoxy-2-(4-methoxyphenyl)-4H-1-benzopyran-4-one (2), 7,8-dihydroxy-2-(2,4-dihydroxyphenyl)-4H-1-benzopyran-4-one (3), 3,7-dihydroxy-2-(4,5-dihydroxy-3-methoxyphenyl)-4H-1-benzopyran-4-one (4),5,6-dihydroxy-2-(4-hydroxyphenyl)-3-methoxy-4H-1-benzopyran-4-one (5), 7-hydroxy-8-methoxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (6), 3,7,8-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (7) and 7,8-dihydroxy-2-(2,3-dihydroxyphenyl)-4H-1-benzopyran-4-one (8), and two new benzoic acids 2-hydroxy-4-methoxy-1,3-benzenedicarboxylicacid (9) and 3,6-dimethoxy-2-hydroxy-benzoic acid (10), found with theknown deoxykaempferol (11), 20,7-dihydroxyflavone (12), 20,3,50,7,8-pentahydroxyflavone(13), 30,40-dihydroxy-3-methoxyflavone (14), 3,40-dihydroxy-3-methoxyflavone (15), galangin(16), gentisic acid (17), isoferulic acid (18), 4-hydroxy-1,2,3-benzenetricarboxylicacid (19), 3,4-dihydroxyphthalic acid (20), 2,5-dimethoxy-benzoic acid (21), p-methoxicinnamicacid (22) and 2,4-dimethoxyisophthalic acid (23). Compounds 11, 12, 13, 14, 15,18, 20, 21 and 23, have not been isolated from plants before.