Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes during Pathogenic Interactions

ROSSI, FRANCO RUBÉN; ROMERO, FERNANDO MATIAS; RUIZ, OSCAR ADOLFO; MARINA, MARÍA; GÁRRIZ, ANDRÉS

Plant Polyamines: Methods and Protocols

Springer-USA

Año: 2018; p. 405 - 416

Plants respond to pathogen attack by modifying defense gene expression and inducing the production ofmyriad proteins and metabolites. Among these responses, polyamine (PA) levels suffer remarkable modifications.Evidences demonstrate that plants make use of the polyamine biosynthetic pathway and theoxidative catabolism of these compounds in order to mount adequate defenses against pathogens. InArabidopsis thaliana, putrescine is synthesized exclusively through the arginine decarboxylase (ADC)pathway, this enzyme exists as two isoforms named ADC1 and ADC2. Even though both isoformsparticipate in the response to pathogen attack, the mechanisms modulating ADC activity are not completelyunderstood. Therefore, studies to clarify their roles are necessary. In this chapter, we describe the methodsthat can be applied for the study of plant?pathogen interactions using Arabidopsis adc mutant plants.