Jasmonic acid and phospholipids involved in the transition of potato stolons to tubers

CENZANO A., RACAGNI G., MACHADO-DOMENECH E., ABDALA G.

Brno, República Checa

Conferencia; 17th International Conference on Plant Growth Substances; 2001

Mendel University of Agriculture and Forestry, Brno

Our purpose was to study the phospholipids (PLs) changes during the morphogenetic transition of stolons in tubers, and to analyze the effect of jasmonic acid (JA) stimulation on the PLs pattern in these organs. Solanum tuberosum L. tubers, cv. Spunta were planted in a greenhouse. Plants grew in a 15 h photoperiod and 30ºC/14ºC day/night. Four stages were collected: stolon, initial of swollen stolon, swollen stolon, tuber set. PLs were extracted, separated by TLC, revealed with phosphomolibdate. No significant differences were found in the PLs among the four stages. The PLs present in stolons and tubers were: phosphatidylinositol bisphosphate (PIP2), phos-phatidylinositol monophosphate (PIP), phosphatidylinositol (PI), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglicerol (PG), phosphatidic acid (PA), cardiolipin (CL), being the most abundant PA. JA stimulation increased PIP2, PIP, PI, PC and PA. Thus, JA seems lo induce gradual changes in the PLs pattern from stolon formation to tuber set, supporting the idea that this compound is involved in the transformation of the stolon into tuber.