Phosphatidic acid produces cell expansion in potato stolons.

CANTORO R, CENZANO A, ABDALA G

Huerta Grande, Córdoba

Jornada; XVI Jornadas Científicas de la Sociedad de Biología de Córdoba; 2007

Sociedad de BiologÍa de Córdoba

Jasmonic acid (JA) and its derivates stimulate potato tuber formation through an increase in central mother cells area of stolonLs apical meristem. JA effect on tuberization could be mediated by phospholipid changes and second messengers production. Cenzano et al. (2007) demonstrated that exogenous JA produces phosphatidic acid (PA) accumulation in the second stage of tuberization. In this work we propose to evaluate PA effect on cell expansion of first and second stage of stolon to tuber transition. For this purpose, two analysis zones located in the apical meristem were defined. Liposomes containing 5 and 10 ƒÊM PA were made for tissue stimulation. Stolons were prepared for microscopic observation and cell area measurement. A statistically significant increase of cell area was caused by 5 ƒÊM PA in Zones I and II of first stage and 10 ƒÊM PA treatment produced the same effect in Zone I of second stage. These results allow us to conclude that PA may act as a lipid messenger during cell expansion process conducing to tuber formation. Moreover, the increase in cell area of apical and subapical zones of first stage stolons suggests that the signal is gtriggeredh in this stage allowing the posterior tuber development.