Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests.

CARBONETTO CH, MALCHIODI EL, CHIARAMONTE MG, DURANTE DE ISOLA EL, FOSSATI CA, MARGNI RA.

CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Año: 1990 vol. 82 p. 93 - 96

0009-9104

By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas´ disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas´ disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.