Induction of Nitric Oxide Synthase after Protein Kinase C Activation by Phorbol Esters

SONSOLES HORTELANO; ANA MARÍA GENARO; MARÍA JOSÉ DIAZ-GUERRA; LISARDO BOSCÁ

Cell Signal Transduction, Second Messengers, and Protein Phosphorylation in Health and Disease

Plenum Press

Lugar: Nueva York; Año: 1994; p. 51 - 64

Activation of Protein Kinase C by biologically active phorbol esters (4ß-phorbol 12,13-dibutyrate or 4ß-phorbol 12-myristate, 13-acetate) promotes the expression of the inducible form of nitric oxide synthase in primary cultures of macrophages and hepatocytes. The induction process is clearly observed after short (hours) incubation periods with phorbol esters, and is antagonized by endotoxins and some cytokines, recognized by their ability to induce oxide nitric synthase, such us lipopolissacharides from the outer wall of Gram negative bacteria. The co-stimulatory effects among cytokines, growth factors, tumor promoters and endotoxins reveal a complex regulatory mechanism in the control of the nitric oxide synthase expression.